Multicolor Brainbow motoraxons in spinoauricularis muscle of adult mouse

Imaged on Olympus FVA using a 40x 1.3NA oil objective and a scan zoom  of 2.4. Four channel sequential acquisition using spectral detectors  to collect CFP and YFP and barrier filters to collect mCherry and Alexa-647. Montage of 6 x 5 tiled image stacks acquired automatically using MATL software.

Image provided by Ryan Draft of Lichtman Lab.